Thermal Cycler Validation
Improve PCR Yields, Control Reagent Usage and Conform to Regulatory Requirements.
The answer could be your thermal cycler.
Anachem brings you Complete Confidence in your PCR data with CYCLERtest PCR thermal cycler validation.
When you programme your PCR thermal cycler, are you sure that it really is achieving the temperature stated, for the required time and that the temperature changes are efficient and uniform across the block? The temperature control is essential for the success of the PCR reaction and the yields obtained.
Anachem now offers the most sophisticated and accurate temperature validation services for your thermal cycler. CYCLERtest provides assurance, with detailed knowledge of how your PCR machine is behaving throughout a reaction. Reporting on the real time performance of PCR machines helps users better understand their results, adapt protocols for more efficient use of valuable reagents and conform to regulatory requirements. For forensic and diagnostic applications complete confidence in the procedure and data obtained is essential and can only be guaranteed with regular checking of the instrument used.
You have two options for your Thermal Cycler Validations. Let Anachem carry out regular on-site testing by a professional technician using the MTAS (Mobile Temperature Acquisition System) or supplement an annual MTAS validation with DIY checks using Driftcon.
MTAS from CYCLERtest
Temperature Validation & Performance TestingThe use of biological positive, negative and threshold controls is insufficient to guarantee reliable PCR results but the unique method of CYCLERtest performs both static and dynamic testing on virtually every commercially available thermal cycler. CYCLERtest uses the MTAS® (Mobile Temperature Acquisition System) that has been calibrated by KEMA (recognised internationally and by UKAS). It is verified by a “Council for Accreditation”. Test results are analysed and reported through an automated process, prohibiting any alterations of the acquired sample data. The Data and layouts are encrypted and cannot be modified. A “Certificate of Thermal Validation” accompanies every test report. This certificate represents the results of the measured and calculated main parameters of the tested instrument. Anachem’s policy is that only trained and certified technicians perform these validations and certifications. Technicians will come to your laboratory for maximum efficiency and convenience and test your thermal cyclers, providing a full report on their functionality. Full understanding of your thermal cycler’s performance will improve optimisation of PCR reactions.
DRIFTCON® – Your Personal Measurement System For Thermal Cycler Validation
The DRIFTCON system is a mobile, personal measurement device that you can use daily, weekly or monthly to monitor the performance and temperature drift of your thermal cyclers. No two thermal cyclers function identically but periodic and continuous validation with DRIFTCON offers a convenient and easy means to check if PCR tests are working within predetermined parameters. Detailed knowledge of your cycler’s true temperature performance can also enable adjustments for improved yields and use of less reagents – saving time and costs.
DRIFTCON measures temperature accuracy- deviation of the actual block temperature from the set temperature. It also monitors uniformity – the spread of the temperature between wells on the same block. It can also provide data on the speed, or ramp rate, at which the cycler changes temperature and report on any under or overshoots – all significant factors affecting the success of your PCR reactions.
Every temperature measurement systems regardless of the thermal cycler model you have, or manufacturer, is performed using a standardized and prescribed temperature protocols, enabling the comparison of results and any deviation from protocol standards.
DRIFTCON temperature results are analysed and reported through an automated process, prohibiting any alterations of the acquired sample data, helping you keep a record of all your results.
To find out more about validating your thermal cyclers or purchasing a DRIFTCON system please Contact Us , Call: +44 (0) 1582 745080 or email pipetteservice@anachem.co.uk
